Journal article

Publication year: 2021

Journal: Journal of Chromatography A

Volume number: 1635

ISSN: 0021-9673

eISSN: 1873-3778

Open access status: Hybrid

DOI Link: https://doi.org/10.1016/j.chroma.2020.461727

Publisher: Elsevier

Abstract: 

Giant goldenrod (Solidago gigantea Ait.) root extract was screened for bioactive compounds by high-performance thin-layer chromatography (HPTLC), coupled with effect-directed analysis including antibacterial (Bacillus subtilis F1276, B. subtilis subsp. spizizenii, Aliivibrio fischeri and Xanthomonas euvesicatoria), antifungal (Fusarium avenaceum) and enzyme inhibition (acetyland butyrylcholinesterases, alpha- and beta-glucosidases and alpha-amylase) assays. Compounds of six multipotent zones (Sg1-Sg6) were characterized by HPTLC-heated electrospray ionization-high-resolution mass spectrometry (HRMS) and HPTLC-Direct Analysis in Real Time-HRMS. Apart from zone Sg3, containing three compounds, a single characteristic compound was detectable in each bioactive zone. The bioassay-guided isolation using preparative-scale flash chromatography and high-performance liquid chromatography provided eight compounds that were identified by NMR spectroscopy as clerodane diterpenes. All isolates possessed inhibiting activity against at least one of the tested microorganisms.

Harvard Citation style: Moricz, A., Krüzselyi, D., Ott, P., Garadi, Z., Beni, S., Morlock, G., et al. (2021) Bioactive clerodane diterpenes of giant goldenrod (Solidago gigantea Ait.) root extract, Journal of Chromatography A, 1635, Article 461727. https://doi.org/10.1016/j.chroma.2020.461727

APA Citation style: Moricz, A., Krüzselyi, D., Ott, P., Garadi, Z., Beni, S., Morlock, G., & Bakonyi, J. (2021). Bioactive clerodane diterpenes of giant goldenrod (Solidago gigantea Ait.) root extract. Journal of Chromatography A. 1635, Article 461727. https://doi.org/10.1016/j.chroma.2020.461727